Measurement

Part:BBa_K1055001:Design

Designed by: Kai Fenzl   Group: iGEM13_TU_Darmstadt   (2013-09-18)


LssmOrange is a fluorophor derived from dsRed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide sequence was added in order to avoid the formation of secondary structures during gBLOCK assembly and subsequent steps. The amino acid sequence was left unchanged.


Source

IDT Technologies, gBLOCK assembly and subsequent PCR isolation

References